Probiotic bacteria with natural fungicide properties for pathogen control

Overview

This offering centers on a proprietary library of probiotic bacteria and eukaryotic cells (fungi and insects) that produce powerful antimicrobial compounds through natural, non-GMO evolutionary gene and pathway insertions. These microbes have demonstrated the ability to kill fungal pathogens individually or as consortia, making them valuable for agricultural pathogen control, seed treatment, and soil protection applications. The approach combines microbial screening, genome sequencing, and compound identification to deliver consistent, biologically derived fungicide solutions.

Technical specifications

Key features:

• Library of bacteria, fungi, and insect cell cultures with validated antimicrobial activity
• Biofilm-based and liquid co-culture systems for pathogen inhibition testing
• Genome sequencing revealing novel genes linked to antimicrobial production
• HPLC-UV chromatographic profiling identifying candidate peaks matching plant terpenoids
• Peak intensity correlated with gene expression, culture conditions, and antimicrobial outcomes
• Polymicrobial and multi-cellular biofilm models for biodynamic pathogen prevention
• Two development pathways: non-GMO in vitro speciation and host-adaptation, plus genetic engineering using stable cassettes for antimicrobial expression
• Culture age, species combinations, and gene expression identified as critical variables for consistent antimicrobial outcomes

Technology readiness level

The technology is at an advanced research and validation stage. Screening tests have demonstrated microbial growth under varied conditions, with cultures and extracts successfully used as plant and soil sprays and seed inoculants. Antimicrobial validation has shown that select microbes and biofilms kill fungal pathogens, and certain insect cell cultures resist contamination in antimicrobial-free conditions. Genome sequencing and HPLC-UV analysis have already identified novel genes and candidate antimicrobial peaks. Future validation will focus on growing cells under specific conditions including co-culture with Fusarium and other pathogens, purifying and testing compounds of interest against industry-selected pathogens, and cloning target genes into safe fertilizer microbes for assessment as living coatings that produce protective molecules.