Promoter variants for enhanced flowering gene expression in brassicaceae
This research focuses on developing promoter variants to increase the expression of a flowering gene, aiming for earlier flowering in Brassicaceae. The approach involves genome editing and transgenic lines to facilitate the introduction of beneficial mutations.
Overview
The research aims to enhance the expression of a flowering-promoting gene through the development of suitable promoter variants. By focusing on the CO-FT module, which is conserved in Brassicaceae, the project seeks to advance the timing of flowering by shifting the expression of the CONSTANS (CO) gene earlier in the day. This strategy is expected to result in transgenic lines that exhibit dominant mutations for earlier flowering, particularly in intermediate photoperiods.
Technical specifications
- Conserved Module: Utilizes the CO-FT module, critical for discriminating photoperiods in plant species like Arabidopsis and Brassica.
- Genome Editing Approach: Involves editing promoters of BnaCO.A10 and BnaCO.C9 genes to relieve repression and increase CO gene expression.
- Transgenic Lines: Arabidopsis lines complemented with BnaCO.A10 and BnaCO.C9 will be edited to observe early flowering traits.
- Screening and Validation: The process includes cloning, transformation, and screening of early flowering lines, followed by sequencing to identify novel haplotypes.
Technology readiness level
The project is at Technology Readiness Level 4, indicating that technology components have been validated in a lab environment. Future validation steps include cloning, transformation, and backcrossing to determine the flowering effect in Brassica napus.
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