RBP32 RNA-splicing protein inhibition for enhanced crop resistance to Phytophthora pathogens
A plant biotechnology approach targeting the conserved RNA-splicing protein RBP32 in Solanaceae crops (tomato, potato, pepper) to enhance resistance against Phytophthora capsici and Phytophthora infestans. Validated through gene silencing and overexpression studies, with chemical inhibitor screening underway to develop practical crop protection solutions.
Overview
This research addresses a critical challenge in Solanaceae crop production by targeting RBP32, a conserved RNA-splicing protein, to enhance plant resistance against devastating Phytophthora pathogens. Phytophthora capsici and Phytophthora infestans cause significant yield losses in tomato, potato, and pepper crops worldwide. The approach offers a novel molecular target for crop protection, with potential applications in seed treatment, foliar sprays, and integrated pest management programs.
Technical specifications
Key findings supporting the approach:
- Silencing the RBP32 gene in tobacco and tomato confers resistance to both Phytophthora capsici and Phytophthora infestans
- Overexpressing wild-type tomato RBP32 in N. benthamiana enhances plant susceptibility to both pathogens
- Overexpressing an RNA-binding-deficient mutant of RBP32 fails to enhance susceptibility, indicating that the RNA-binding activity of RBP32 is essential for pathogen compatibility
- The mechanism appears conserved across Solanaceae species, suggesting broad applicability
Future development pathway:
- Screening chemical inhibitors that specifically target RBP32 function
- Testing selected compounds on tomato, potato, and pepper crops
- Evaluating efficacy against both P. capsici and P. infestans under controlled and field conditions
Technology readiness level
The technology is currently at an early-to-mid stage of development. The genetic validation has been completed through gene silencing and overexpression experiments in model and crop plants. The next phase involves chemical inhibitor screening and application testing across multiple Solanaceae crops. This represents a transition from molecular proof-of-concept toward practical agricultural applications, with potential for partnership in chemical development, field validation, and crop protection product commercialization.
About Nanjing Agricultural University
Nanjing Agricultural University is a public research university with an agricultural focus in Nanjing, advancing national and regional priorities through integrated basic-to-applied research and field training. Industry engages through experimental farms, field stations, controlled-environment facilities, and pilot processing spaces for prototyping and scale‑up. An industry liaison team supports sponsored research, testing services, and joint labs, while regional demonstration sites speed on‑farm trials across the Yangtze River Delta. Research is supported by national and provincial programs, including the National Natural Science Foundation of China and the Ministry of Science and Technology. A technology transfer office manages IP, licensing, and startup incubation.
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