Real-time DNA/RNA detection using sensor and microfluidic nanotechnology

Overview

This technology is a stimuli-responsive polymer-based optical sensing platform designed to perform rapid and reliable analysis of DNA and RNA targets with high sensitivity and accuracy. The platform offers a novel approach to nucleic acid detection that is not currently available in the market. By leveraging aptamer or antibody recognition elements immobilized on a gold surface, the device can quantify targets of interest in real time. The approach has already been validated through proof-of-concept detection and quantitation of Tetrahydrocannabinol (THC), demonstrating its potential for broader diagnostic and analytical applications.

Technical specifications

Core design:

• A monolayer of microgel particles is sandwiched between two thin gold films, forming an optical cavity (etalon) that exhibits a visible color
• Recognition elements such as antibodies or aptamers are immobilized on the top gold surface to capture specific targets
• Binding of an analyte to the surface alters the diffusion of a stimulant into the etalon, changing response kinetics proportionally to the amount of analyte bound
• A novel data analysis protocol identifies regions of the etalon array showing the most significant target binding-induced response

Key advantages:

• Rapid, real-time detection capability
• High sensitivity and accuracy for target quantification
• Scalable fabrication processes that can be upscaled to meet commercial demand
• Optimized etalon fabrication protocols improving efficiency and consistency
• Versatile platform adaptable to different targets by changing recognition elements

Technology readiness level

The sensing platform has progressed beyond initial proof of concept, with successful detection and quantitation of THC at industrially relevant levels using optimized aptamer immobilization and binding conditions. Independent verification of binding performance for various aptamers and antibodies has been completed. Current development efforts are focused on improving device sensitivity and evaluating performance in complex matrices such as cannabis extracts. Future work will extend the platform to DNA/RNA detection through capture strand immobilization, followed by validation in spiked solutions, simulated environments, and ultimately real-world samples. A manufacturing facility for commercialization is planned to support beta testing and field validation.