REJOIN-seq for cell-free RNA autoimmunity biomarkers

Overview

REJOIN-seq is an innovative sequencing technology developed by the Institut Pasteur de Montevideo to analyze cell-free RNA (cfRNA) in human fluids. cfRNA molecules circulating in the blood are often fragmented and inherently fragile, causing them to be lost during conventional sequencing processes. REJOIN-seq addresses this challenge by restoring and repairing these partially degraded RNA molecules, allowing researchers to capture and sequence critical biological information that was previously inaccessible. This technology is particularly valuable for identifying upstream triggers of inflammation and cytokine production in autoimmune diseases, as well as for potential applications in the early diagnosis of conditions like cancer.

Technical specifications

• Repairs and restores partially degraded cell-free RNA (cfRNA) for successful sequencing.
• Enables the identification of molecular species typically lost by conventional sequencing methods.
• Effective for analyzing fragile circulating molecules, such as damaged or modified self-RNA and endogenous retroviral RNA, which drive inflammatory cascades.
• Applicable to blood and urine samples for biomedical research and potential diagnostic use.

Technology readiness level

The technology is currently in development and implementation, positioned at TRL 5 or 6. It has moved beyond initial conceptual research into laboratory validation and functional application. The Institut Pasteur de Montevideo is actively applying the technology in collaborative research programs to identify disease-related RNA triggers in autoimmune settings, demonstrating its utility in advanced research environments. Earlier studies on the underlying methodology have been published in peer-reviewed journals, and the technology is being utilized in strategic industry collaborations.