Rhizobium leguminosarum NOD+ for enhanced nodulation and nitrogen fixation

Technology
In development
University

Rhizobium leguminosarum NOD+ is an improved strain offering increased nodulation efficiency and biofilm formation, enhancing nitrogen fixation in crops. It presents a sustainable solution to boost crop yields while reducing nitrogen fertilizer use.

Overview

Rhizobium leguminosarum NOD+ is a cutting-edge inoculant designed to significantly enhance the efficiency of nodulation in legumes, thereby improving nitrogen fixation. This strain has shown a two-fold increase in nodule occupancy compared to its wild-type counterpart in greenhouse assays. The enhanced performance is attributed to the overproduction of an extracellular protein that optimizes the processing of acidic exopolysaccharides, crucial for biofilm formation and effective symbiosis with legumes. By improving host colonization and nodulation efficiency, this solution offers an environmentally friendly and cost-effective way to boost crop yields while reducing reliance on nitrogen fertilizers.

Technical specifications
  • Increased nodulation efficiency: Demonstrated two-fold increase in nodule occupancy in greenhouse assays.
  • Enhanced biofilm formation: Exhibits a three- to four-fold increase in biofilm formation and surface colonization.
  • Extracellular protein production: Overproduction leads to improved processing of exopolysaccharides.
  • Genetic transferability: The trait can be transferred to any R. leguminosarum strain, offering flexibility in strain selection.
  • Field and laboratory validation: Proven in multiple laboratory strains with plans for field trials and further validation.
Technology readiness level

This solution is currently at Technology Readiness Level 4, indicating that it has been validated in laboratory environments and is ready for field trials and further development.


About Instituto Nacional de Tecnología Agropecuaria

INTA is Argentina’s national agricultural technology institute—a decentralized public research and extension organization—with a countrywide network of research centers, experimental stations, and extension units. Co‑located labs and field sites embedded in production regions enable applied R&D and direct engagement through its extension system, offering practical environments for corporate collaboration. A dedicated technology‑linkage function manages licensing, co‑development agreements, and specialized services, while an affiliated foundation can contract and administer international projects to streamline collaboration. INTA is primarily supported by the Government of Argentina under the Secretariat of Bioeconomy, with teams also tapping competitive national and international funding programs. Its technology transfer processes cover IP management, licensing, and support for technology‑based ventures.

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