RT-LAMP lateral flow test for tobamovirus viability detection
A novel RT-LAMP based lateral flow test for detecting live and dead tobamovirus, offering rapid, stable, and multiplexed diagnostics for seed and plant production applications.
Overview
The innovative solution proposed is a Reverse Transcriptase-Loop Mediated Isothermal Amplification (RT-LAMP) based lateral flow test, designed to rapidly detect and differentiate live and dead tobamovirus. This test aims to simplify diagnostics for tobamovirus, a critical concern in seed and plant production, by integrating RT-LAMP with a lateral flow readout. The system promises high stability, adaptability, and multiplexing capability, making it particularly useful in various environmental conditions.
Technical specifications
- RT-LAMP Technology: Utilizes reverse transcriptase-loop mediated amplification to target and amplify tobamovirus RNA.
- Lateral Flow Readout: Provides a simple and rapid visualization of amplification products, indicating the presence of viable virus.
- Intercalating Dye Technology: Incorporates PMA/EMA treatments to distinguish between live and dead virus.
- Adaptability: Can be adjusted to identify other infectious agents important to agriculture.
- Robustness: Designed to function in extreme environments, enhancing its utility in diverse agricultural settings.
Technology readiness level
This technology is currently at TRL 4, having been validated in laboratory settings with known tobamovirus samples. Future validation will involve optimizing primers and testing with inactivated and live virus under controlled conditions to ensure reliability and efficacy.
About Attogene
Attogene is a biotechnology and biomanufacturing company based in Austin, Texas, that specializes in the development and production of rapid detection technologies and molecular biology tools. The company’s portfolio includes lateral flow assays, ELISA kits, qPCR and qLAMP detection kits, and custom assay development services. By leveraging expertise in protein biochemistry, antibody production, and diagnostic assay design, Attogene creates solutions for diverse applications, including environmental monitoring—such as algae toxin and water quality testing—as well as human health, food safety, and pathogen detection. Their approach focuses on making complex scientific analysis more accessible through portable and efficient diagnostic formats.
These products and services are designed to support researchers, laboratories, and industrial partners in fields ranging from environmental protection to clinical diagnostics. The company assists clients with project needs from early-stage feasibility and prototype optimization through to manufacturing and quality validation. By providing tools that reduce the time, cost, and complexity associated with traditional testing methods, Attogene enables more effective process control and environmental oversight. The company is also active in research and development, including projects supported by agencies such as the National Institutes of Health (NIH), to advance innovations in RNA therapeutics and diagnostic technologies.
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