Rt-qpcr assay for distinguishing viable pathogens in vegetables
A laboratory-based RT-qPCR assay that targets species-specific mRNA to differentiate viable from non-viable tobamoviruses in solanaceous seeds, offering high sensitivity and specificity within 3-5 hours, suitable for phytosanitary and regulatory compliance.
Overview
The RT-qPCR assay is an innovative solution designed to distinguish viable from non-viable tobamoviruses in solanaceous seeds using species-specific mRNA as a marker. Unlike traditional DNA-based detection methods, this approach leverages the rapid degradation of mRNA upon cell death, ensuring accurate identification of pathogen viability. This method provides high sensitivity and specificity, delivering results within 3–5 hours, and is compatible with routine laboratory workflows. The assay supports scalable and reliable seed health testing, aligning with phytosanitary and regulatory standards.
Technical specifications
- Species-specific mRNA targeting: Ensures detection of viable pathogens only, reducing false positives associated with DNA-based methods.
- Rapid assay results: Capable of delivering results within 3-5 hours, facilitating quick decision-making.
- High sensitivity and specificity: Proven repeatability and discriminatory power, demonstrated in preliminary studies on foodborne pathogens.
- Scalability: PCR-based method avoids hazardous reagents, making it suitable for large-scale applications.
- Compatibility: Works within existing molecular diagnostic frameworks, enhancing practicality for seed health testing.
Technology readiness level
The technology is currently at TRL 4, indicating that it has been validated in a laboratory environment. Future validation steps include primer and probe design, RNA extraction optimization, and scalability assessments. The project benefits from the advanced qPCR systems and molecular diagnostic expertise available at Texas A&M University, positioning it for further development and industry adoption.
About Texas A&M University, College Station
Texas A&M University in College Station is a comprehensive public research university and the flagship of The Texas A&M University System, combining broad academic strengths with a strong applied‑research culture. Industry collaborates on the Texas A&M‑RELLIS campus—an integrated education, research and testing environment that supports large‑scale experimentation and proving grounds—and through the Texas A&M Transportation Institute’s facilities in Bryan‑College Station. A statewide extension network connects university expertise to companies and communities across all Texas counties, enabling rapid piloting and deployment. Research is supported by competitive federal funding from agencies such as NSF, NIH, DOE, USDA and DoD, alongside state and industry sponsorship. Texas A&M Innovation provides IP management, licensing and commercialization pathways across the system.
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