Synergistic multi-microorganism bioproduct for plant-pathogenic nematode control
A combined bioproduct leveraging three complementary microorganisms (nematophagous fungus Duddingtonia flagrans, fungus Trichoderma koningiopsis, and rhizobacteria Bacillus amyloliquefaciens) to synergistically control the plant-pathogenic nematode Ditylenchus dipsaci while preserving biodiversity. Builds on individually validated biocontrol agents repurposed for integrated phytonematode management.
Overview
This research proposes a novel bioproduct that combines three microorganisms with complementary modes of action to control the plant-pathogenic nematode Ditylenchus dipsaci in a way that protects soil biodiversity. The formulation brings together Duddingtonia flagrans, a nematophagous fungus that captures nematode larvae through three-dimensional adhesive traps; Trichoderma koningiopsis, a fungus known for parasitism, enzymatic lysis, antibiosis, and competition against pathogens; and Bacillus amyloliquefaciens, an endophytic rhizobacterium that colonizes plant tissues and produces metabolites with nematicidal activity. Each component has a track record in other agricultural applications, and the project seeks to determine whether their combination produces a synergistic effect against phytonematodes.
The value proposition for industry partners is a biologically based, biodiversity-friendly alternative to chemical nematicides for crops affected by D. dipsaci, such as vegetables and other susceptible horticultural species. By repurposing and combining existing biocontrol agents, the approach aims to reduce dependence on synthetic pesticides while supporting sustainable crop production.
Technical specifications
Mechanism of action of each component:
- Duddingtonia flagrans: Captures nematode larvae through three-dimensional mycelial traps, physically reducing nematode populations in the rhizosphere
- Trichoderma koningiopsis: Acts through multiple complementary modes including parasitism, enzymatic lysis of nematode structures, antibiosis through secondary metabolites, and competition for space and nutrients
- Bacillus amyloliquefaciens: Endophytic rhizobacterium that colonizes plant tissues internally and secretes metabolites with nematicidal properties
Proposed validation activities:
- Standardization of a laboratory bioassay testing different developmental stages and population densities of Ditylenchus dipsaci
- Individual evaluation of each microorganism against the target nematode
- Evaluation of binary and ternary mixtures to detect synergistic interactions
- Assessment of microbiological compatibility among the three strains to ensure stable co-formulation
- Plant-model trials contingent on positive laboratory results
Technology readiness level
This proposal is at an early research stage (TRL 2–3). The individual microorganisms have established efficacy in other agricultural applications, including gastrointestinal nematode capture, plant growth promotion, and metabolite-based pathogen suppression, but their combined performance against Ditylenchus dipsaci has not yet been validated. The current project is designed to generate the foundational laboratory evidence needed to justify development of a combined bioproduct, with downstream plant-model testing planned as a next step based on initial results.
About Corporación Colombiana de Investigación Agropecuaria
AGROSAVIA is a Colombian decentralized public entity of mixed non-profit participation dedicated to agricultural science, technology, and innovation. The organization focuses on generating scientific knowledge and developing agricultural technology through research, technological adaptation, and the dissemination of technical expertise. Its operations are organized into specialized innovation networks, including cacao, fruits, vegetables, livestock, permanent crops, and roots and tubers. By bridging the gap between advanced research and practical application, AGROSAVIA develops improved crop varieties, such as biofortified beans and sugarcane, and establishes integrated management protocols to enhance agricultural productivity and sustainability.
These efforts are vital for Colombian agricultural producers, providing them with the tools and knowledge necessary to improve competitiveness, ensure sustainable natural resource management, and raise their quality of life. Through initiatives like field schools and the provision of technical assistance, the corporation facilitates the transfer of technology and information to small-scale farmers. By fostering collaboration between the public and private sectors, AGROSAVIA acts as a key driver for technical change in the national agricultural sector, helping producers modernize their practices and address challenges related to climate resilience and food security.
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