Targeted discovery of insecticides for ion channels in brown planthopper
Innovative research to identify effective insecticides targeting ion channels in resistant and non-resistant brown planthopper strains, addressing agricultural pest control challenges.
Overview
This research proposal focuses on the targeted discovery of insecticides that act on ion channels in the brown planthopper (BPH), an agricultural pest affecting rice crops. The study aims to understand the molecular basis of insecticide resistance in BPH by examining over 20 ion channel genes that are potential targets for insecticide action. This research is crucial for developing effective pest control strategies, as current insecticides are becoming less effective due to evolved resistance in BPH populations.
Technical specifications
- Phase 1: mRNA sequencing to identify channel variants in resistant BPH strains; cloning and electrophysiology to test functional differences in channels like NaV, KV, CaV, iGluRs, GABAA, and AChRs; comparative pharmacology to assess insecticide sensitivity; structural docking to understand reduced drug binding due to amino acid substitutions.
- Phase 2: Development of stable cell lines expressing target and resistant channel variants; establishment of a high-throughput screening pipeline to identify novel compounds; integration of electrophysiology with docking studies to elucidate block mechanisms.
Technology readiness level
The project is currently at Technology Readiness Level 2, involving experimental proof of concept with ongoing functional, structural, and pharmacological studies. The research is in collaboration with the University of Toronto's Lunenfeld Center for high-throughput screening and aims to develop a pipeline for novel insecticide discovery and efficacy testing.
About University of Toronto
The University of Toronto is a comprehensive public research university with three campuses in the Toronto region and a globally scaled research enterprise. Its downtown footprint is embedded within a major academic health network and an adjacent innovation district, enabling co-located labs, clinical trials, and rapid testing with end users. Companies connect through co-op and long-duration internships, sponsored research, and access to shared core facilities and prototyping resources. Research is supported by Canada’s Tri‑Agency (NSERC, CIHR, SSHRC) and the Canada Foundation for Innovation, alongside provincial programs and industry partnerships. A dedicated technology transfer office provides IP management, licensing, and startup support through a coordinated entrepreneurship network.
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