Waste-derived acetate to terpenoid ingredients via engineered Y. lipolytica

Overview

The proposed solution is a biomanufacturing platform leveraging engineered Yarrowia lipolytica to convert waste-derived acetate into valuable terpenoid ingredients. This sustainable approach uses acetate, obtained through CO₂ electrochemical conversion, gas fermentation, or industrial waste streams, as a feedstock. It bypasses the use of food-competing sugar feedstocks, aligning with sustainability goals and cost-effectiveness. The platform demonstrates industrial scalability and product diversification across various terpenoid classes, making it suitable for applications in fragrances, bio-actives, and polymer precursors.

Technical specifications

The engineered strains of Yarrowia lipolytica utilize a native high-flux mevalonate pathway and robust acetate metabolism to produce terpenoids such as squalene, geraniol, and mevalonate. The process has achieved significant yields: 2.3 g/L squalene, 1.1 g/L geraniol, and 1.8 g/L mevalonate from acetate as the sole carbon source. The ability to swap terpene synthases allows for modular product diversification, enhancing the platform's flexibility and applicability. Future work includes expanding the terpenoid product range and optimizing the fermentation process through precision techniques to further reduce production costs.

Technology readiness level

Currently, the technology stands at TRL 4, indicating that it has been validated in a laboratory setting. Future steps involve scaling up the process to pilot-level fermentation, with plans to advance to commodity-scale production. This includes optimizing fermentation conditions and demonstrating commercial viability through pilot trials, in collaboration with Minnesota Biomanufacturing Services.