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Seed health tests for pathogen viability
  • Background
  • What we're looking for
  • What we can offer you
  • Who we are
  • Q&A
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Background

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Plantum is a consortium of companies active in plant breeding, seed production, and propagation, working collectively to support market access and strengthen phytosanitary standards across the seed sector. BASF and Bayer, aligned with Plantum’s mission, have joined forces to explore innovative solutions that improve seed health testing, leveraging their combined expertise in seed technology, virology, and regulatory insight. 

 

Providing healthy (vegetable) seeds to customers is a prerequisite for sustainable food production. A healthy seed is free from pathogenic organisms that could cause disease in the developing plant. Frequently used methods to determine seed health such as ELISA and PCR detect the presence of proteins or nucleic acids associated with specific plant pathogens. However, these methods cannot distinguish between viable, infectious pathogens and non-viable remnants. As a result, a positive test may indicate the presence of non-viable organisms, residual genetic material left on the seed after a disinfection treatment, or environmental contamination with genetic material, rather than an actual infection risk. Therefore, such test results may not reliably reflect the true risk posed by a seed lot when it is brought into the market. 

 

Seed companies contribute to the availability of healthy seeds by routinely testing them to prevent or control plant pests and pathogens that may affect seed quality, seed movement, and their introduction into new territories. Good management practices that prevent seeds from exposure to pests and diseases throughout all stages of seed development, production, and commercialization significantly reduce overall pest risks, and seed health testing is often a final check. A seed health test is also frequently required by National Plant Protection Organizations (NPPOs) as a condition for importing seed into their territories.

What we're looking for

We are looking for a seed health test method, whether destructive or non-destructive, that distinguishes between the presence of viable and non-viable pathogens in or on the seed. The primary focus is on testing for tobamoviruses in solanaceous crops, such as tomato, pepper, and eggplant.

This could be a new confirmation test or a completely different method that shows the viability of pathogens present in/on the seed. We are especially interested in creative and unconventional approaches beyond those listed below.

Solutions of interest include:
  • Viability PCR using propidium monoazide (PMA) or ethidium monoazide (EMA)
  • Seed bioassay combined with molecular marker detection
  • Immunocapture with viability dye or protease treatment
  • Enzyme-based viability detection
Our must-have requirements are:
  • Designed for use in a laboratory environment
  • Strong rationale for achieving sensitivity comparable or higher than PCR
  • Test turnaround time of up to 7 days, with preliminary data or rationale supporting repeatability
Our nice-to-have's are:
  • PCR-based test
  • Easy adjustment of the test/method for other crops and other viruses
  • Cost considerations available (e.g., estimated project costs, anticipated cost per sample)
  • Scalability for routine use
What's out of scope:
  • Crop-pest combinations other than tobamoviruses in solanaceous crops
  • Methods requiring high-risk chemicals that may pose safety hazards to employees
  • Solutions blocked by third-party intellectual property rights
Acceptable technology readiness levels (TRL):
Levels 1-9
What we can offer you
Eligible partnership models:
Sponsored research
Benefits:
Sponsored Research
Initial funding is available to support proof-of-concept studies or feasibility data collection, with the potential for follow-on funding depending on preliminary results and project scope.
Facilities and Services
Partners can send samples for analysis at our facilities.
Expertise
Partners will have access to an internal team of experts as appropriate.
Data
Partners can leverage the data set for additional insights regarding the solution.
Who we are

As a global chemical company, we operate in diverse markets with varying requirements. To ensure responsible conduct and maintain our license to operate, we are guided by our core values and standards. These values shape our daily actions and foster respectful relationships with colleagues, customers, and partners.

Our teams are committed to putting health and safety first, making sustainability a part of every decision, and meeting strict compliance and environmental standards. We embrace diversity—in people, perspectives, and experiences—and promote open feedback grounded in honesty, respect, and trust. We see setbacks as opportunities to learn and improve.

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Reviewers
Q&A with BASF

The Q&A is now closed.

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Q.
Would a non-PCR, lab-based electrochemical sensor that detects the difference between viable and non-viable tobamoviruses within 10 minutes be considered in scope for your seed health testing call? Thanks.
4
A.
Thank you, Jonathan. We are definitely interested in your approach. Please submit a proposal!
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Emir Islamovic, Innovation and Partnership Manager, BASF
May 22, 2025
Is this response helpful?
0
0
Q.
For this project, how long will it be? What is the total funding range?
3
A.
Thank you for the question. Our desired outcome for the initial collaboration is a proof-of-concept. The length required to achieve that PoC may differ. We are further interested in follow-on collaborations if the initial engagement is successful and mutually beneficial. In short, it will depend on the proposal.
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Emir Islamovic, Innovation and Partnership Manager, BASF
May 22, 2025
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0
0
Q.
Would the consortium consider a solution detecting ~100 targets/sample with viability pretreatment, PCR-based amplification, quantified output, and an automation-ready <4h long workflow validated outside seed health?
2
A.
Thank you for the question. It depends on the technology you would bring. For example, PMA is known from literature. If it is something novel, it would certainly be of interest. If quantified output can be correlated to biological activity, we are interested.
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Emir Islamovic, Innovation and Partnership Manager, BASF
May 22, 2025
Is this response helpful?
0
0
Q.
Would a non-PCR method using near-infrared spectroscopy (portable or benchtop instruments) be within the scope of the problem?
1
A.
Thank you for the question. Yes, non-PCR method using near-infrared spectroscopy would be within the scope.
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Emir Islamovic, Innovation and Partnership Manager, BASF
June 2, 2025
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0
0
Q.
Hi. Are optical imaging/spectroscopic technologies within the scope? Would you provide samples for testing?
1
A.
Yes, such technologies are in scope and we could provide samples for testing.
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Emir Islamovic, Innovation and Partnership Manager, BASF
June 18, 2025
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0
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