Crispr-based rapid on-field detection platform for plant GMO characterization

Overview

KRYSP-R is developing a CRISPR-based rapid on-field detection platform designed to transform how genetically modified organisms (GMOs) are characterized in agricultural environments. Traditional GMO detection methods, such as PCR and sequencing, are often time-consuming, expensive, and require specialized laboratory equipment and trained personnel. This innovation addresses those limitations by combining CRISPR's precision nucleic acid recognition with lateral flow assay (LFA) technology to create a portable, user-friendly diagnostic tool.

The platform targets both point mutations and large gene insertions in plant GMOs, delivering highly sensitive and specific detection suitable for precision agriculture, regulatory compliance, and contamination prevention. By enabling real-time, on-site decision-making, the technology helps farmers, seed producers, and regulatory agencies reduce costs and accelerate workflows.

Technical specifications

• CRISPR-Cas detection system utilizing Cas12a or Cas13a-based assays for sensitive and specific nucleic acid recognition
• Rapid sample processing through a modified buffer system designed for fast and efficient DNA/RNA isolation without specialized lab equipment
• Recombinase Polymerase Amplification (RPA) integration to enable isothermal amplification suitable for field conditions
• Lateral flow strip readout providing visual results within 30-40 minutes from sample collection to detection
• Portable, all-in-one device design integrating sample preparation, nucleic acid extraction, amplification, and CRISPR-based detection into a single platform
• Customizable probe design targeting known point mutations and large gene insertions specific to individual GMO events
• Provisional patent filed to protect the underlying intellectual property

Technology readiness level

The technology is currently at an early-to-mid development stage. KRYSP-R has validated a similar CRISPR-based point-of-care detection system for plant pathogens, demonstrating fluorescence-based detection at varying limits of detection (LOD) through in-silico probe design and in-vitro cleavage efficiency assessments. The next milestone is the development of an all-in-one lateral flow strip-based detection system for plant pathogens, which will serve as a foundation for extending the platform to GMO detection. Future validation plans include designing GMO-specific CRISPR probes, building a portable integrated device, benchmarking against PCR and sequencing for sensitivity and specificity, optimizing to minimize false positives and negatives, and conducting field trials across diverse growing conditions and GMO varieties.