Detection of mycotoxins in grain extracts using HPLC and LC-MS
Innovative method for detecting mycotoxins in grain extracts utilizing HPLC and LC-MS technologies, capable of identifying compounds with and without chromophores through pre-concentration and derivatization techniques.
Overview
This solution offers an advanced method for detecting mycotoxins in grain extracts through the use of High-Performance Liquid Chromatography (HPLC) and Liquid Chromatography-Mass Spectrometry (LC-MS). Mycotoxins, harmful compounds produced by fungi, are often found in food products, and detecting them at low concentrations is critical for food safety. This method effectively identifies mycotoxins with chromophores using HPLC UV-vis DAD, and those without chromophores through pre-column derivatization or LC-MS, ensuring comprehensive detection capabilities.
Technical specifications
Key features:
- Utilizes HPLC UV-vis DAD for detecting mycotoxins with chromophores.
- Employs pre-column derivatization or LC-MS for mycotoxins without chromophores like Fumonisin.
- Pre-concentration techniques include organic extraction and Solid Phase Extraction (SPE) enrichment, enhancing detection sensitivity by 10 to 100 times.
- Steps involve homogeneous grinding, organic solvent extraction, nitrogen or frozen drying, and re-dissolution into mobile phase for analysis.
- Capable of detecting mycotoxins at concentrations as low as 0.XX-XXX ppb (ug/Kg).
Technology readiness level
This method is currently at Technology Readiness Level 3, indicating it has been demonstrated in laboratory settings and is in the early stages of validation. Future plans include further testing and validation using organic extraction or SPE enrichment, followed by direct testing or derivatization and LC-MS analysis.
About Pacific Biolabs Inc
Pacific BioLabs is a contract research organization (CRO) providing life science testing services for the medical device, pharmaceutical, and biotechnology industries. Founded in 1982 and headquartered in Hercules, California, the company specializes in preclinical and regulatory studies, including toxicology, biocompatibility, pharmacology, analytical chemistry, sterility assurance, and microbiology. Its operations are built upon a quality system aligned with current Good Manufacturing Practices (cGMP) and Good Laboratory Practice (GLP) regulations, and the laboratory maintains ISO/IEC 17025:2017 accreditation to ensure technical competence and compliance with international standards.
These services support clients in navigating the complex regulatory requirements for product safety and development submissions. By providing specialized testing capabilities, Pacific BioLabs helps manufacturers meet FDA and EPA expectations for product validation and quality control. The company operates a purpose-built facility where it performs a wide range of analytical and biological tests, serving as an outsourced partner for organizations that require rigorous scientific assessment to advance their life science products from development through regulatory approval.
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