High-speed LC-MS/MS detection method for mycotoxins in cereal grains
This innovative LC-MS/MS method efficiently detects five major mycotoxins in cereal grains, including deoxynivalenol and aflatoxin B1. It uses optimized chromatography for high sensitivity and rapid throughput in the low ppb range, ensuring precise quantification.
Overview
Our solution offers a cutting-edge high-speed LC-MS/MS method for the detection and quantification of five major mycotoxins in cereal grains, including deoxynivalenol, ochratoxin, fumonisin, zearalenone, and aflatoxin B1. These mycotoxins, which are harmful to human and animal health, can now be detected with exceptional precision and speed, thanks to our advanced chromatography techniques. The method is designed for high throughput and operates in the low parts-per-billion (ppb) range, ensuring accurate results even in complex grain matrices.
Technical specifications
- Chromatography Optimization: Utilizes a combination of C18 and HILIC columns to identify the best stationary phases and solvent systems for rapid, sensitive detection.
- Target Mycotoxins: Focuses on sesquiterpene epoxides and polyketides, with special attention to ochratoxin A's unique nitrogen and chlorine components.
- Sensitivity and Throughput: Achieves low ppb detection limits, allowing for high sample throughput and reliable quantification.
- Extraction Procedures: Evaluates a range of solvent polarities to optimize recovery and selectivity, improving quality control through internal standards.
Technology readiness level
Currently at TRL 4, this technology has undergone validation under laboratory conditions with controlled samples. Future work includes further optimization and validation in real-world grain matrix conditions to advance to higher TRLs.
About University of Toronto
The University of Toronto is a comprehensive public research university with three campuses in the Toronto region and a globally scaled research enterprise. Its downtown footprint is embedded within a major academic health network and an adjacent innovation district, enabling co-located labs, clinical trials, and rapid testing with end users. Companies connect through co-op and long-duration internships, sponsored research, and access to shared core facilities and prototyping resources. Research is supported by Canada’s Tri‑Agency (NSERC, CIHR, SSHRC) and the Canada Foundation for Innovation, alongside provincial programs and industry partnerships. A dedicated technology transfer office provides IP management, licensing, and startup support through a coordinated entrepreneurship network.
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