Nanobiosensor for real-time detection of lipoperoxides in biological samples
Innovative nanobiosensor for non-invasive, real-time detection of lipoperoxides, offering high specificity and sensitivity. Suitable for assessing food quality and guiding preservation strategies.
Overview
The nanobiosensor developed by our lab offers a groundbreaking approach for the real-time, non-invasive detection of lipoperoxides in biological samples. This sensor is particularly valuable for assessing the quality of food products by detecting early markers of lipid peroxidation, such as precursors of hexanal. Early detection helps in maintaining product quality and can effectively guide preservation strategies, making it a significant tool in the food industry.
Technical specifications
Our nanobiosensor is based on fluorescent proteins that allow for the detection of lipoperoxides with unprecedented specificity and sensitivity—over 100 times higher than traditional hydrogen peroxide detection methods. The sensor operates with a high sensitivity at nanomolar concentrations and provides rapid results within seconds. The assay is cost-effective, adaptable to 96 or 384-well formats, and requires only a standard fluorescence plate reader, eliminating the need for expensive equipment or specialized technical expertise. It is also autocalibrated, ensuring consistent performance across different assays.
Technology readiness level
Currently at Technology Readiness Level 5, the nanobiosensor has been functionally validated in vitro and in cellula. We plan to further validate its application in detecting lipoperoxides in specific sample types such as dry animal proteins or other relevant food products. This will involve close collaboration with industry partners to customize and optimize the assay to meet specific quality and applicability standards.
About Institut Pasteur de Montevideo
Institut Pasteur de Montevideo is a specialized biomedical research foundation with roughly 200 scientists and an international outlook, designed to connect biology with health and biotechnology value. Its purpose-built site shares a campus with the University of the Republic’s Faculty of Sciences, supporting close collaboration, training, and access to advanced infrastructure. Shared technological platforms and laboratories serve researchers inside and outside the institute and national and regional productive sectors. An innovation and technology-transfer function, innovation space, and BIOESPINN incubator support commercialization and startup formation. Funding includes institutional support and external awards from Uruguay’s ANII and INIA, MERCOSUR’s FOCEM, the Inter-American Development Bank, and the Pasteur Network.
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