Lamp-based rapid detection kit for viable pathogens in seeds
A field-deployable, low-cost diagnostic kit using LAMP technology to detect viable seed-borne pathogens by targeting mRNA, providing rapid, accurate detection of fungi, bacteria, and viruses. Ideal for crop biosecurity and seed certification.
Overview
The LAMP-based rapid detection kit is an innovative diagnostic tool designed for the agricultural sector, specifically to enhance biosecurity and seed certification processes. Utilizing Loop-mediated Isothermal Amplification (LAMP) technology, this kit targets mRNA to identify viable pathogens in seeds, ensuring rapid and accurate detection of fungi, bacteria, and viruses. By differentiating between live and dead pathogens, it offers a significant advantage over traditional detection methods. Its portable and easy-to-use design makes it ideal for on-site use, addressing the urgent need for real-time pathogen monitoring in diverse agricultural settings.
Technical specifications
- Technology: Loop-mediated Isothermal Amplification (LAMP)
- Target: mRNA of viable pathogens
- Pathogens detected: Fungi, bacteria, and viruses
- Detection method: Colorimetric or fluorescent
- Applications: Seed certification, crop biosecurity, disease prevention
- Features: Portable, field-deployable, low-cost, user-friendly
Technology readiness level
Currently at Technology Readiness Level 3, this solution is in the early stages of development. The research involves selecting seed-borne pathogens, designing primers for mRNA targets, and optimizing LAMP assays. Future phases will focus on the development and field validation of a portable diagnostic kit, followed by scaling up production and establishing distribution channels.
About South Dakota State University
South Dakota State University is a comprehensive public land‑grant research university serving a large statewide community. Industry engages through a university‑linked research and technology park that colocates tenant companies with faculty labs and pilot‑scale processing suites for de‑risking and demonstration. A statewide extension network and field sites provide real‑world environments for validation and market feedback, while shared‑use core facilities and tailored workforce pipelines connect talent and tools to corporate R&D. Research is supported by competitive federal funding from USDA and NSF, with additional awards from NIH and DOE, alongside state and industry partnerships. A dedicated technology transfer office manages IP and industry agreements, and incubator space at the research park supports startup formation and scale‑up.
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