Novel diagnostic assay for detecting viable pathogens in seeds
A cutting-edge diagnostic assay using Loop-Mediated Isothermal Amplification (LAMP) combined with DNase for rapid, on-site detection of viable pathogens in seeds, offering a safe and efficient alternative to traditional methods.
Overview
The novel diagnostic assay developed by PathoScan leverages the Loop-Mediated Isothermal Amplification (LAMP) technique combined with DNase to provide an efficient and safe method for detecting viable pathogens in plant tissues, including seeds. This innovative approach enables the rapid identification of seed contamination directly at the farm site, eliminating the need for laboratory hazardous waste disposal associated with traditional methods. The assay's on-site application potential makes it ideal for producers to monitor and manage seed health, ensuring quality and safety.
Technical specifications
- Utilizes LAMP technology for rapid amplification of target DNA sequences
- Integrates DNase to degrade DNA from dead cells, ensuring only viable pathogen detection
- Simplified procedure: involves lysis, heating, and dipstick steps for easy use
- Designed for use with a variety of plant tissues, including seeds
- Safe and non-toxic alternative to PMA-based assays
Technology readiness level
This diagnostic assay is at Technology Readiness Level 4. It has been validated in controlled laboratory settings and is undergoing further validation through field trials to establish its efficacy and reliability in real-world conditions.
About Pathoscan.com
PathoScan Technologies Inc. is an agricultural technology company based in Saskatoon, Canada, that specializes in rapid, on-site crop disease detection. Founded in 2022 by Ethan Done, Tayab Soomro, and Erik Tetland, the company developed a portable diagnostic system—comprising the PathoBox and PathoKit—that enables agronomists and growers to test for plant pathogens in the field. By utilizing techniques such as Loop-Mediated Isothermal Amplification (LAMP) alongside DNA amplification and artificial intelligence, the system provides lab-quality diagnostic results within hours, significantly reducing the traditional turnaround time of several days or weeks. The company focuses on serving sectors like greenhouse operations, targeting pathogens such as Botrytis and mildew.
This technology addresses critical pain points for producers by enabling proactive crop management and informed decision-making based on real-time data. By making sophisticated diagnostic technology portable, affordable, and user-friendly, PathoScan aims to bridge the gap between complex lab-based testing and field-level applications. The company emphasizes a made-in-Canada approach to hardware manufacturing and has engaged with the Saskatchewan agricultural community and regional innovation programs to support its development and market entry. Its solutions offer a practical tool for farmers and agronomists seeking to minimize crop losses through early, reliable pathogen detection.
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